The study of genetic diversity in Foeniculum vulgare using ISSR molecular marker

Fennel (Foeniculum vulgare Mill.) is one of the well-known medicinal and aromatic plants. To determine variability between 16 Iranian Fennel ecotypes based on molecular markers, an experiment was carried out based on completely randomized design with three replications at Payame Noor University of Kermanshah, Iran, in 2014. Ecotypes were evaluated based on 15 ISSR primer markers. All of the ISSR primers showed 77 visible bands. The ISSR13 and ISSR10 primers had the most number of bands with 10 and 9 bands respectively. The mean polymorphic information content (PIC=0.36), marker index (MI= 1.83), Effective multiplex ratio (EMR= 4.98) and polymorphism percentage (91%) indices were calculated for all primers. Total genetic similarity based on these primers was 0.567 percent. Cluster analysis classified all ecotypes to three groups. This clustering was confirmed by principal coordinate (PCo) and analysis of molecular variance (AMOVA). Genotypes were evaluated based on 15 ISSR primer markers. All of the ISSR primers showed 77 visible bands. The ISSR13 and ISSR10 primers had the most number of bands with 10 and 9 bands respectively. The mean polymorphic information content (PIC=0.36), marker index (MI= 1.83), Effective multiplex ratio (EMR= 4.98) and polymorphism percentage (91%) indices were calculated for all primers. Total genetic similarity based on these primers was 0.567 percent.