Investigation for Preparation and production of Radio-Kit Vitamin B12 with Med-grade (1.Report)

Labelled vitamin B12 with 58 Co  is one of the important ingredients of the diagnostic Kits for diagnosis of patients affected by anemia. Insufficient absorbtion of vit. B12 is one of the causes anemia in humans and one of the accurate methods for measurement of absorbed vitamin B12 is application ofvit. B12 with radioisotope cobalt – 58 labelled. The isolation and purificationof labelled vit.B12 from fermentation medium of Streptomyces Olivaceus essential process for kit prepration.. The first experiment of this research was isolation and purification with using different resins with solution of cyanocobalamin containing cobalt-59 that was produced by this bacterium.  After investigation and pre-feasibility experiments two non polar resins XAD-4 and XAD-7 were selected. The results of the different experiments on XAD-4 and XAD-7 showed that XAD-4 is much better than the latter one for immobilization of cobalamin. The isolation of vitamin B12 on XAD-4 column was achieved by different solvents such as methanol, ethanol and isopropanol in the different ratio concentrations. The results of TLC (Thin Layer Chromatography) showed that the best eluants solution for desorbtion of cobalamin from XAD- 4 column are solvents such as methanol, ethanol and isopropanol with maximum concentration of vitamin B12 in the range of concentrations 25 up 50 percent, 30 up 40 per-cent and 10 per-cent respectively. Cobalamin was collected in aceton and crystallized in low temprature. .