Novel automated technique for determination of multiresidue and multiclass antibiotics in chicken tissue by Liquid chromatography tandem mass spectrometry

Today, the use of antibiotics for the prevention, control and treatment of poultry industry is unavoidable and on the other hand requiring a modern approach that providing high sensitivity and specificity, convenient and rapid method to detection antibiotics. Therefore, in this study, for the first time, a wide range of antibiotics used in poultry industry were studied and monitored based on liquid chromatography techniques. Therefore, two liquid chromatography-mass spectrometers were used to prepare the samples with acetic acid and acetonitrile and z-sep+ compounds and with internal standards of antibiotic groups of sulfonamides, quinolones and tetracycline and comprised the results with conventional common method. Because of using of polymeric interface, absorption a large number of antibiotics was possible, so a wide range of antibiotics could be measured without any setting back in static phase efficiency. At the preparation, the amount of sample used is lower than that of classical methods and the preparation steps get shorter. Steady isotope of internal standard caused controls in the extraction, purification, injection and calculations of possible errors such as ion subsidence, injection error, percent recovery of drug and retention time correction and also the possibility to inject high volumes and increase the sensitivity in this method was improved. Therefore, exploitation two liquid chromatography devices, one for sample preparation provides big advantages, which alone lacks this feature. Meanwhile, simultaneously automate method reduces analysis time, test costs and increases accuracy compared to classical method and the sample preparation gets very convenient and allows the ability to search for antibiotics in the produced green chicken.