Investigation of antioxidant and antibacterial characteristics of Hawthorn's (Crataegus monogyna) wood ethanol and acetone extracts

The aim of the current study was to investigate the antioxidant activity of Hawthorn's woods ethanol and acetone extracts and the effect of their antimicrobial on Escherichia coli, Staphylococcous aureus and Bacillus cereus bacteria. Extraction was performed by maceration method with two acetone and ethanol (96 percent) solvents at a ratio of 1:20 of sample to solvent. Total phenol content was measured by Folin– Ciocalteu method, and also the antioxidant properties were measured. Antibacterial activity of the extracts was determined at 0.7, 0.8, 0.9 and 1 percent (v/v) concentrations by well diffusion and test broth macro dilution method, respectively. The results showed that TPC and the percentage of inhibition of DPPH and ABTS free radicals of acetone extract were higher than methanol extract, and the highest effect on the diameter of the non–growth zone was related to 1 percent (v/v) of both extracts. Also, the results of antibacterial tests showed that the minimum inhibitory bactericidal were obtained with 1 percent of the extracts against B. cereus. The results of GC/MS also showed several functional compounds in the acetone extract.