Impact of different quantity of Zinc oxide nanoparticles on growth and hematology of Mrigal Cirrhinus mrigala

Zinc is essential for aquatic biota including fishes at a lower concentration, but when it reaches higher concentration it becomes toxic. The objectives of the present work were related to the impact of different quantities of zinc oxide nanoparticles on the growth and hematology of Mrigal Cirrhinus mrigala. The zinc oxide nanoparticles were synthesized by chemical precipitation method and characterized using by UV-VIS, SEM, EDAX, FTIR and XRD. Different quantity of zinc oxide nanoparticles such as 0, 5,10,15,20 and 25mg/100g were prepared by using a fish meal, groundnut oil cake, wheat flour, and tapioca flour. Feed utilization and hematological parameters of Mrigal were estimated after 21 days of feeding. UV-visible adsorption spectra show that the peak absorbance of ZnO nanoparticles was observed 500 nm. SEM shows that nanoparticles formed are clustered because of the adhesive nature of flower-shaped appearance. EDAX shows that the zinc oxide nanoparticles and the peaks are located between 1.0Kev and 8.5Kev. The FTIR spectrum of zinc oxide nanoparticles was analyzed in the range of 400-4000cm-1 and spectral bands were observed. The XRD results were viewed as the crystalline nature and average size of zinc oxide nanoparticles. Survival rate indicated that all Mrigal were healthy during the period of 21 days except in feed II,IV, and V. The feed utilization and growth parameters are higher in feed IV. Hematological parameters such as hemoglobin, RBC, Hematocrit, MCV, MCH, MCHC of Mrigal progressively increased and WBC and platelets decreased with increase in the quantity of Zinc Oxide nanoparticles.