The Effect of Collagen on the Synthesis of Calcium Phosphate Nanoparticles

Collagen and Hydroxyapatite (HA) nanoparticles are significant constituent of the natural bone. In this study, the effect of collagen on the morphological and phase characteristics of calcium phosphate nanoparticles was investigated. The synthesis reaction was initiated by mixing H3PO4 as phosphorous source and CaCl2 as calcium source in presence of Collagen Type 1. Collagen concentration in suspension and Ca to P ratio was 1% and 1.67 respectively. The morphology and structure of samples (with collagen and without collagen), heat treated at 600 0C were characterized by X-Ray diffraction (XRD), Fourier transformation infrared (FTIR) and Scanning electron microscopy (SEM). More fine and flake-like shape particles were observed in the SEM images of sample synthesized in the presence of collagen compared to the control sample which was constituted of larger granular particles. The XRD results revealed the powders were composed of hydroxyapatite and octacalcium phosphate and the sample synthesized in the presence of collagen was less crystalline. The amide bands of collagen and P-O and OH characteristic peaks were identified in FT-IR spectra.