The effect of 15 and 30 militesla static magnetic field on the rat neuronal induced mesenchymal stem cell, cell cycle

The effect of 15 and 30 mT of static magnetic field on the cell cycle of neural inductive rat BMSC was evaluated. The BMSC was inductived by neural inductive medium (NIM). Duration of inductive and Exposure time were 2, 4 and 6 hours. The cells induction to neural inductive medium associated with SMF as exposed group, besides we have a control group. The apparatus we used to generate the SMF was a locally designed SMF generator in which there was an incubator instrument capable to maintain the humidity, temperature and CO2 concentration in predefined level. After exposing, the cells were fixed, stained and their percent of cell cycle phases; G1, S, G2/M were elucidated using flowcytometer instrument. The WinMdi 2.9 Software was used to process data from flowcytometer and elucidation of phase's percents. The results showed SMF with 15 mT intensity did not significantly alter the cell cycle in three different (2, 4 and 6 hours) exposing times. Exposing 2 hours with 30 mT increased the G2/M phases in neural inductive BMSC comparing to the corresponding control. Static magnetic field decreased the percent of S phase in BMSC, during 4 hours exposure.