Cytotoxic effects study of nano-selenium oxide-enriched Saccharomyces bullardi on induced breast cancer cells by DMBA in rat

This research aimed to study the anticancer effects of nano-selenium oxide-enriched Saccharomyces bullardi on DMBA-induced breast cancer cells in rats.

In order to induce cancer, the carcinogen with a concentration of 60 mg/kg was injected into the left breast nipple of the female rats. When the tumors grew to about 10 mm, the cancerous tumor was isolated from the breast of the animals and used to make tissue sections and individual cells. Cancer cells were treated with different concentrations of S. boulardi suspension and nano-selenium oxide-enriched S. boulardi. The cytotoxicity assay was performed with two methods of MTT and Trypan blue staining.

Comparison between two cell groups treated with S. boulardii and nano-selenium oxide-enriched S. boulardi using two tests, it was found that there were significant changes in cancer cells viability in both 0.5,1 µg/ml concentrations. with increasing the concentration, the cancer cell viability was significantly decreased. cell viability in the cell line treated with nano-selenium oxide-enriched S. boulardi yeast, with the increase in the concentration of enriched yeast was decreased. A comparison between the yeast and enriched yeast in different concentrations, revealed that there was a significant difference between cytotoxicity effects of them on the cells.

Probiotic yeasts could be an appropriate candidate for the treatment of some diseases including cancers, especially when combined with selenium compounds.