Genetic variation study of Teucrium polium genotypes in the west of Iran using SCoT molecular markers

Teucrium polium L. is one of the well-known medicinal and aromatic plants. In this study, we have used the SCoT marker to determine genetic variation among 17 genotypes of Teucrium polium collected from Kermanshah province, Iran. All of the SCoT primers showed 48 polymorphic bands. The primers of Sc20, Sc59 and Sc21 showed the highest number of band (6 bands) and Sc15 showed the lowest number of bands (2 bands). The mean polymorphic information content (PIC=0.361), marker index (MI= 1.525), Effective multiplex ratio (EMR= 4.197), polymorphism percentage (98.485%) and Resolving Power (RP=2.837) indices were calculated for all primers. The greatest similarity was between Gilan-gharb2 and Salas2 stations with 0.732 values. The lowest genetic similarity was between Naft-Shahr with Gilan-gharb1 and Gilan-gharb2 stations with 0.1. The cluster analysis group showed that the genotypes were classified into two groups, and the results of the clustering were confirmed by the use of molecular variance analysis (AMOVA) and principal components analysis (PCoA).