A Simple ELISA System for Detection of hIFN-y

The use of accu rate, rapid, cheap and sensitive methods for measurement of cytokines in body fluids is absolute prerequisite to detine involvement of these mediators in various clinieal situations and pharmacological effect of recombinant cytokines administration. In this study a sandwich enzyme-linked immunosorbent assay (ELISA) was designed for detection of small amounts of human interferon gamma (hIFN-y). Alter immunization of a female New Zealand White (NZW) rabbit and a female BALB/c mouse against recombinant human IFN-y, a high level titer of antibody was produced that confirmed by dot blot technique. A precipitated antibody from rabbit serum was used as tirst antibody and mouse serum used as second antibody. By determination of the best antibody concentrations and optimization of other conditions, an ELISA system was designed. The data indicate that this ELISA was efficient and sensitive for detection of as little as 40nglml of recombinant hIFN-y.