Why Should TREC and KREC Quantification Assay be Concerned about Screen of Newborns in Developing Countries?

Primary immunodeficiencies contain a set of several different diseases. Giving the fact that their clinical outcome ranges from mild to potentially life-threatening, detecting the patients with these diseases in the neonatal period is considered as the main goal of efforts that are currently being made. It has been reported that T-cell receptor excision circles (TRECs) and kappa-deleting recombination excision circles (KRECs) are circular DNA segments produced in T and B cells during their maturation in the thymus and bone marrow, respectively. Fortunately, excision circles can be reliably quantified using real-time quantitative PCR techniques.