IIn vitro Evaluation of Microleakage of Universal Bonding for Fissure Sealing of Permanent Mandibular Teeth

Fissure sealing is considered one of the most important preventive methods of caries in dentistry. This study aimed to investigate the effect of universal bonding on fissure sealing of permanent molars.

This experimental study was performed on 30 healthy human molar teeth that were divided into three groups, namely 1) fissure sealing by total bonding and acid etching, 2) fissure sealing by universal bonding and acid etching, and 3) fissure sealing by universal bonding without acid etching. After the preparation of the buccolingual section of the teeth, the microleakage was evaluated quantitatively. The collected data were analyzed in SPSS software (version 20) using Kruskal-Wallis and Dan tests.

In this microleakage study with the dye penetration method, the highest and lowest degrees were observed in the groups of universal bonding without etching and the total bonding with etching, respectively. It must be noted that the difference was significant between these two groups (P=0.001). There was also a significant difference between the two groups of universal bonding without etching and universal bonding with etching (P=0.003)

Regarding the microleakage in the universal bonding group, it is better to use a combination of acid etching and universal bonding in fissure sealant. The degree of microleakage was lower in the two groups of fissure sealing by total bonding with acid etching and universal bonding with acid etching, compared to the group of fissure sealing by universal bonding alone.