The Survey of anticancer and anti Microbial properties of Rosmarinus officinalis L, Peganum harmala L and Coffee Extracts on cell viability of Cancer cells and Pathogenic Bacteria in Foods

Despite significant advances in radiation therapy and cancer treatments in the past 30 years, resistance to chemotherapy is a major obstacle in the recovery of patients with cancer. Resistance to chemotherapy drugs inhibits the recovery process. This study aims to evaluate the anticancer activity of the hydroalcolic extracts of Rosemary, Peganum, and Coffee on growth inhibition of MCF-7 cell line and determine the rate of growth inhibition of pathogenic bacteria isolated from food. We examined cytotoxicity effects of three different concentrations (60,120,250 μg/ml) of Rosemary, Peganum, and Coffee extracts on MCF-7and HDF cell lines. The cell lines were grown in RPMI supplemented with 10% FBS, 1% penicillin, and streptomycin. The cells were allowed to incubate at 37ºC in an atmosphere that contained 5% CO2 and 95% humidity. The standard MTT assay was performed to estimate cell viability after treatment by Rosemary, Peganum, and Coffee extracts. The extracts were also used with MIC on Staphylococcus aureus and E. coli bacteria isolated from food. The results of the MTT assay showed that the Rosemary, Peganum and Coffee extracts had time-and concentration-dependent anticancer activities on the MCF-7cell line compared to HDF cells statistically significant (p<0.01). As the Rosemary extract had more effect on the control of cell proliferation than the other two extracts. Also, these extracts at different concentrations had an inhibitory effect on food pathogenic bacterial growth. Our results have suggested that these plants extract is a potential candidate, either alone at pre-determined doses, for the dietary supplement suitable for the inhibitory effect on food pathogenic bacterial growth and cancer cell lines.