Separate and the survey of biological activity from fractions of venom the Persian Gulf Stichodactyla haddoni

S. haddoni is one of the venomous animals of Persian Gulf and characterization of its biological activity and toxicity are of great value concerning discovery of new potential pharmaceutical agents as well as study of toxicity of the crude venom. The main goal of this study was to survey biological activities fractions isolated from methanol extract of tentacles from the Persian Gulf sea anemone. LD50 of isolated fractions venom of S. haddoni was recorded on Balb/c mice with intravenous injection. Edematogenic activity of isolated fractions from venom was performed by injection into the sub plantar region of the left hind paw male Balb/c mice (average weight 20–22 g) in tetra plicate. Dermonecrosis activity of the venom was checked on male New Zealand rabbit's skin (3kg). LD50 of F1 was recorded at 38.8 mg/ml. The highest edematogenic activity was seen just two hours after injection 5mg/ml from F4 (179.4 %) and decreased up to 70.5% during 10day. 30mg/ml of F1, F3 and F4 fractions made necrosis area 4, 3, 5mm respectively after 3 hours. Instant potent edema and rapid dermonecrotic activity are significant phenomena after injection of intradermal. This is the first report on instant edematogenic and rapid dermonecrotic activities of the Persian Gulf Sea Anemone venom, Stichodactyla haddoni. The results would also be of high value to better management of clinical conditions and follow up treatment strategies as well.