Role of KATP channels and vitamin E on Acute convulsion induced by PTZ in rats

In this study the role of vitamin E and KATP channels and corelation between them has been evaluated on acute induced chemical convulsion by PTZ in rats.

36 male wistar rats were divided into six groups with six rats. In control group, animals experienced convulsion by PTZ (60 mg/kg, i.p. injection, in acute model). Rats in other groups were injected by vitamin E (E group), Diazoxide (Diazoxide group), 5-Hydroxy Decanoic Acid (5HD group), and binary compound of this drugs (E,Diaz and E,5HD groups) half hours before seizuring. Seizure parameters including time of stage 2 , time of stage 5 , duration of stage 5 and finally seizure stage has been measured after interference of drug 30 minute prior to PTZ injection in different groups.

in every three stages of convulsion in two groups treating by vitamin E and Diazoxide has been increased significantly comparing to control group. There were no significant change in all stages comparing to control group and the group which has taken both E,Diaz and E,5HD groups shown no significant change in favor of seizure control by E vitamin in presence of blockers or openers of KATP channels.

This study indicates effective vitamin E and Diazoxide role by individual in control of seizure but rejected this vitamin direct relationship with KATP channel and also reject vitamin E cooperative role and KATP channels in acute seizure control that show synergist effect for them.