The effects of sub-lethal doses of copper sulfate in zebra fish ovary tissue

Copper is one of the heavy metals used in aquaculture for the elimination of algae, as well as prevention and treatment of fish diseases. The effects of sub-lethal doses of copper sulfate in zebra fish ovary tissue were investigated in 30-day exposure. For this purpose, 270 females with average weight of 0.3±0.05 were subjected to 0, 0.02 and 0.04 mg/l cuso4. At the end trail, samplings were done from gonad tissue and the tissue sections were prepared. Results showed that in the control group, ovary tissue was normal and major of the oocytes were sexually at the beginning and end of fourth stage. In 0.02 mg/l oocytes were in the second and third stage. In 0.04 mg/l, the slight growth of ovarian was observed and oocytes were in the second stages and a number of oocytes were observed at the beginning of third stage and atrotic follicles were observed in gonad tissue. This process shows an increase in egg growth with increasing copper sulfate concentration.