The effect of probiotic supplementation on motor skills, biochemical factors and histological evaluation in a multiple sclerosis model of mouse

Multiple Sclerosis (MS) is an autoimmune, inflammatory, and demyelinating disease of the central nervous system (CNS) leading to some disorders including gait, balance, memory, cognition as well as pain and fatigue. This study aimed to evaluate the therapeutic effects of probiotics on behavioral disorders caused by disease and biomarkers of oxidative stress in mice fed with cuprizone.

In this study 45 male C57BL/6 mice were randomly divided inti 4 groups: control group; control + probiotic group; cuprizone group and cuprizone + probiotic group. Treatment group were fed a diet containing 0.2% cuprizone to create an animal model of MS.

The results of this study showed that in the motor behavior test, both healthy and MS groups receiving probiotics remained longer on the rota rod during some days of the experiment. However, probiotics could not reduce the oxidative indices in these animals.

Probiotic supplement can have a mild effect on improving motor phenomena and antioxidant indices in healthy or MS animals. However, to ensure that different doses of cuprizone and probiotics are needed, changes in the animal model and intervention are made.