Molecular detection of the species Ehrlichia ewingii among dogs referred to veterinary clinics in Semnan, Iran

In recent years, there has been a growing interest in research into the Anaplasmataceae family. In this family, there is the genus Ehrlichia, which is a compulsive intracellular and zoonotic bacterium. The main carriers of these bacteria are mites, the most common species of hard ticks that are found mainly in all parts of the world, such as Ixodes, Dermacentor, Rhipicephalus and Amblyomma. The aim of this study was to track the molecular detection of Ehrlichia ewingii among referred dogs referred to the veterinary clinic of Semnan University. In this study, 134 dogs were randomly assigned to each of them in the amount of 4-5ml of blood and they were used to extract DNA using phenol-chloroform method. Subsequently, with the help of Anaplasmataceae primers, the desired gene was traced and positive cases were recorded. Based on the results, out of the total number of reference samples, 35 samples were positive, of which 21 were male and 14 were female but none of them were Ehrlichia positive after the second round of PCR.