Evaluation of Bacteriovorus Stalpii predator on the healing of Pseudomonas aeruginosa-infected burn wounds in rats

Drug-resistant infection is one of the leading causes of death from burn injuries. Bacteriovorus stalpii, like other predators, invades and destroys the periplasmic space of gram-negative bacteria. The aim of the present study was to investigate the Bacteriovorus stalpii on the healing rate of Pseudomonas aeruginosa-infected burn wounds in rats.

30 albino rats were randomly divided into 5 groups of positive, negative, gentamicin, ciprofloxacin and staphylococcal bacteriorex after 3 burn wounds.30 rats N-MRI were randomly divided in to 5 groups positive and negative control, gentamicin, ciprofloxacin and Bacteriovorus stalpii, and except for the negative control group, all groups Burned and Infected by 4 multidrug resistant Pseudomonas isolates. After treatment with bacteria and antibiotics, wound colonies were reported every other day and wound area was measured within 20 days.

The results of colony count showed that Bacteriovorus stalpii in infected wounds significantly reduced the amount of bacteria in 21 days compared to other groups and this decrease was better than the performance of gentamicin and ciprofloxacin antibiotics. Measurement of wound area over 20 days also showed that the use of predatory bacteria reduced wound area in burnt rat compared to control groups. Histological examination of the resulting wounds showed that the predatory bacteria reduced inflammation, increased fibroblasts cell and increased tissue collagen compared to the control group.

Bacteriovorus stalpii can accelerate the treatment process in drug-resistant infections.