Effect of methanol foliar application on expression changes of some mitochondrial genes in canola under drought stress

In this study, two canola genotypes, Hyola308 (susceptible to drought) and SLM046 (tolerant to drought) were evaluated for expression of thioredoxin H (TRX H), alternativa oxidase 1 (AOX1), pyruvate dehydrogenase 1 (PDH1), isocitrate dehydrogenase 1 (IDH1) and fumarase 1 (FUM1) genes using real-time PCR technique under three conditions: non-drought stress, regular irrigation and methanol solutions sprayafter 72 hours irrigation interruption. PDH gene expression was the highest in SLM046 at zero stress (72 h after irrigation), IDH at 8 h after methanol spray, FUM at 24 h after methanol spray, TRX at 8 h after methanol spray, and AOX at 24 hours after sampling. In Hyola308 genotype, expression of PDH, FUM, TRX, and AOX genes increased 2, 25, 6, 1.4 and 5 folds, respectively after 8 hours of foliar application and IDH gene expression was high at zero hour of sampling. Overall, methanol foliar application increased the expression of the examined genes, which were significantly higher in SLM046 than Hyola308 genotypes.