Immunohistochemically detection of ATP-sensitive potassium channel proteins and mitochondrial biogenesis in the heart tissue of male rats after 8 weeks of increasing interval training

From the point of view of molecular studies, the effect of interval training on the expression of ATP-sensitive potassium channel proteins and proteins related to mitochondrial biogenesis has not been studied. Therefore, the present study aimed to immunohistochemically ATP-sensitive potassium channel proteins and mitochondrial biogenesis in the heart tissue of male rats after 8 weeks of interval exercise.

The present study was an experimental study in which samples were composed of 10 healthy male Wistar rats (5 weeks old and mean weight 150 70 70) which were randomly divided into control and interval exercise groups. Exercise group periodically performed the: interval exercise protocol for 8 weeks. External cardiac tissue and levels of mitochondrial biogenesis proteins (TFAM, PGC1α) and ATP-sensitive potassium channels (KIR6.2, SUR) were measured by immunohistochemically tests. One-way analysis of variance and Tukey's post hoc test was used to analyze the data. Significance level was considered less than 0.05.

After 8 weeks of interval exercise, it was shown that the interval training group had a significant difference in terms of expression of (TFAM, PGC1α) and KIR6.2, SUR compared to the control group (P = 0.001).

It seems that interval exercise appears to increase the number of ATP-sensitive potassium channels in the heart tissue of mice, which in turn increases mitochondrial biogenesis in heart tissue.