Effect of drought stress, soil salinity, P.indica and MWCNs on bio-degradation of diesel oil in the Pb and Cd polluted soil under cultivation of triticale plant

Today, soil pollution with heavy metals and petroleum compounds is one of the main environmental problems. This research was done to investigate the effect of drought stress, soil salinity, piriformospora indica (P.indica) and MWCNs on bio-degradation of diesel oil in the soil that contaminated with Pb and Cd under cultivation of triticale plant. Treatments consisted of applying MWCNs (2% (W/W)) in the Pb and Cd polluted soil that was simultaneously polluted with diesel oil (0, 5 and 10 % (W/W)) and the plants used in this study was inoculated with P.indica. After 70 days, plants were harvested and plant and soil Cd were measured using AAS. In addition, the degradation percentage of diesel oil in soil was determined. Soil application of MWCNs at the rate of 2 % (W/W) significantly increased the bio-degradation of diesel oil in the soil in the drought and salinity (6 dS/m) stress by 11.3 and 15.6%, respectively. In addition plant inoculation with P.indica significantly increased the bio-degradation of diesel oil in the soil by 12.3%. For salinity (6 dS/m) and drought stress, it was increased by 8.3 and 9.4%, respectively. In addition, the Pb and Cd concentration was decreased by 14.3 and 12.8, respectively, when the MWCNs was added to the soil (2 % (W/W)). Soil application of MWCNs (2 % (W/W)) and plant inoculation with P.indica not only increased the bio-degradation of diesel oil, but also decreased the plant Pb and Cd concentration that is a positive point in the environmental studies.