The study of active compounds and antioxidant activity of waste residues obtained during Essential oil and Rose water production in 24 Damask Rose Populations of East and West Azerbaijan: By-product

In this research, the amount of phenolic compounds and antioxidant activity were measured in waste obtained during producing of essential oil and rose water process and compared this compounds with methanolic extract of petals which are the main source of these compounds. 24 populations of Damask rose were collected from 11 cities in both East and West Azerbaijan provinces. Extracting essential oil and rose water were done in each of samples and finally, all wastes were sampled and methanolic extract of petals were obtained based on usual way. Total phenol of samples was measured by Folin-Ciocalteu method and total amount of flavonoid was determined by Aluminum chloride colorimeter method. The antioxidant activity of samples was also measured in two way, 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) and Iron reduction. The obtained results in all measured factors were significant (p> 0.01). The obtained values of total phenol showed that the wastes can be used to extract of 44.5% of total phenol that extracted by methanolic extraction. In total flavonoid, obtained values of wastes showed that about 92% of total flavonoid in methanolic extract can be extracted from wastes. The measured antioxidant activity also showed that waste samples had the activity range of 0.14-1.30 mmol Fe++ /g DW in iron reduce method; in DPPH they also inactivated 17 to 71% free radicals. According to the results of this research, waste of essential oil and rose water producing can be used as a suitable and cheap resource to obtain natural antioxidants.