Preparation of Composites of Antibacterial ‎Materials Based on Bacterial Cellulose ‎and Silver Nanoparticles for Wound ‎Healing

This study examined the preparation of composites of Bacterial Cellulose (BC) / silver nanoparticles (Ag NPs) using a substrate for the fermentation of the bacteria Gluconacetobacter xylinus C-3 and a reducing agent for the synthesis of silver nanoparticles in situ. The presence of silver in the BC/ Ag NPs composites was confirmed by various characterization tests. Thus, according to Scanning Electron Microscopy, the average size of silver was recorded at 700 nm. X-ray phase analysis showed an increase in the size of cellulose crystallites up to 122 Å with a decrease in the amount of silver nanoparticles. According to UV-visible spectroscopy, an identification peak was found between 420-580 nm. Measurements of the percentage of water absorption and the edge angles of wetting with water confirmed the structures of the resulting nanocomposites - with a smaller pore diameter and a narrow pore distribution over the film size, depending on the silver content. Using the disk diffusion method, excellent antimicrobial activity was shown with a decrease in the number of bacteria in relation to the pathogenic microorganism Staphylococcus (S.) aureus, Pseudomonas (P.) aeruginosa, Escherichia (E.) coli.