Network Analysis of 20S-Ginsenoside Rg3 Effect on Human Colorectal Adenocarcinoma Cell Line HT-29

20S-Ginsenoside Rg3 is a pharmacological active compound of ginseng. Evidences indicate that S20-Rg3 as an anti-cancer factor plays role in prevention and treatment of cancer. In the present study, proteomic data of 20S-ginsenoside Rg3 effect on human colorectal adenocarcinoma cell line HT-29 was analyzed via network analysis to understand more details about the molecular events. The differentially expressed proteins (DEPs) related to the effect of 20S-ginsenoside Rg3 on human colorectal adenocarcinoma cell line HT-29 were extracted from literature and analyzed via protein-protein interaction (PPI) network. The central nodes of the network were determined based on degree value and betweenness centrality. Eight DEPS plus 100 added first neighbors were included in the PPI network. Five central nodes as hub-bottlenecks including ACTB, GAPDH, TP53, AKT1, and ALB among the added first neighbors and ANXA5 as hub-bottleneck and GSTP1 and PCNA as bottlenecks among the queried DEPs were introduced. PCNA, GSTP1, and ANxA5 as cell protective proteins are the crucial targeted proteins by 20S-ginsenoside Rg3 in the treated cell line HT-29. Up-regulation of GSTP1 and ANXA5 is correspondent to the cell protective property of 20S-ginsenoside Rg3, and down-regulation of PCNA refers to the opposite effect. It seems that cell protective roles of 20S-ginsenoside Rg3 are accompanied with the possible side effects.