Extraction and identification of Gallic acid and some active ingredients in Lavandula angustifolia plant and synthesis of silver nanoparticles by green method from the extract of this plant

In this research, the flowering branches of shami Lavandula angustifolia plant, from the mountains of Ilam province and the flowering branches of Larestani Lavandula angustifolia plant from Bushehr province were collected, dried and ground in the shade. Their methanolic and aqueous-methanolic extractive were extracted by maceration, soxhlet and ultrasonic methods and some important compounds were identified by the GC-MS method. Due to the large variety of extractives from Lavandula angustifolia extract, column chromatography, plate chromatography, thin layer chromatography and crystallization were used to separate the important combination of gallic acid from methanolic extract. The compound was finally purified as a white powder and identified by spectroscopic methods, and a high-performance liquid chromatography (HPLC) device was used to determine the percentage of Gallic acid. The silver nanoparticles were then prepared by green method from Lavandula angustifolia extract and examined in silver nitrate solution using UV-vis spectroscopy in the wavelength range of 200 to 800 nm. Also, Field Emission Scanning Electron Microscope (FE-SEM) for particle morphology, XRD for the particle scattering pattern and with FT-IR device, the functional groups responsible for reducing and stabilizing silver nanoparticles were evaluated.