Investigating and describing the method of diagnosis of spore of Nosma disease in several apiaries in Ilam province

The disease of Nosma is from a mature bee, caused by the Nosema spp. An opportunistic agent that forms spores inside the cell and expands rapidly as it leaves the cell, and is recognized as an important insect pathogen. Nozma is one of the most serious fungal diseases of mature honey bees, which is present all over the world, and is created by two species of nosma apis and nozma serana, and it spreads to all three bees in the hive. The purpose of this study was to describe how to determine the type of spore of the disease of Nozma caused by the development of this disease, which was carried out in several apiaries in the province of Ilam. In this research, a sample of adult bees from a number of apiaries was taken up by 70% alcohol by a glass of alcohol content and sent to the Microbiology Laboratory of the Faculty , University of Ilam. Consequently, Nosma spores were observed with the help of a microscope, in form of large, elliptical, white and bright and uniform. The number of spores in the honey bee was counted and determined using a special formula. A common drug in the past has been fomaglin. Therefore, fomagelin is used in Iran and prevents the development of Nozma.