In Vitro Synergistic Effects of Ciprofloxacin, Vitamin E, And Low Power Laser on Human Dermal Fibroblasts

Human Dermal Fibroblasts (HDF) are involved in the production of the extracellular matrix, formation of the connective tissue, and wound healing. Considering the role of ciprofloxacin in the treatment of skin infections and the subsequent oxidative stress as well as the protective effects of vitamin E and low power laser against inflammation and oxidative stress, we evaluated combined effects of low power laser and vitamin E on inflammation and oxidative stress in HDF cells treated with ciprofloxacin.

Morphology of the cells was studied using an inverted microscope. Viability of the cells was assessed using the MTT assay, and the concentration of reactive oxygen species was determined after exposure of the cells to ciprofloxacin (5, 25, 50, 75, and 100 μg/ml), vitamin E (1 mg/ml), and low power laser (660 nm; power density: 30 mW.cm−2).

The survival rate of the cells increased significantly after the treatment with ciprofloxacin, vitamin E, and low power laser compared with the cells treated with ciprofloxacin and vitamin E (p<0.001). The amount of reactive oxygen species increased in the treated cells when compared with those only treated with ciprofloxacin and vitamin E.

The low power laser treatment has favorable effects on the growth of HDF cells, which can be beneficial for wound healing, even in the presence of ciprofloxacin.