EVALUATION OF THE ROLE OF HYDROALCOHOLIC EXTRACT OF GINGER (ZINGIBER OFFICINALE L.) AND VITAMIN C ON CELL VIABILITY OF NORMAL GINGIVAL AND SKIN CELLS IN THE PRESENCE OF DRABKIN’S SOLUTION

In today's society, chemicals have many benefits and applications. One of these chemicals is Drabkin’s solution which is used in the hematology laboratory. Drabkin’s solution is toxic and is harmful to skin health. In this study, considering the chemical composition of vitamin C and the phytochemical properties of ginger plant, we decided to investigate the effects of the hydroalcoholic solution of this plant on the survival of normal skin and gum cells in the presence of Drabkin solution.

Protective effects of ginger extract and vitamin C (doses 0.1, 10, 50, 100, 500, and 1000 μM) on the toxicity caused by Drabkin’s solution was measured by colorimetric assay (MTT) to investigate the survival and viability of normal gingival and skin cells. Prism statistical software version 8 and one-way ANOVA and Tukey tests were used for statistical analysis. A significance level of P<0.05 was considered.

The results of this study showed that high concentrations of vitamin C and ginger inhibit the toxicity of Drabkin’s solution, so that there was a significant increase in the growth of normalized cells. Drabkin’s solution in concentrations higher than 50 μM has toxicity on the studied cell lines.

The results of this study showed that the consumption of ginger extract and vitamin C in different concentrations increased the growth of normal cells of the gums and skin, and as a result, caused cell protection from the damage caused by Drabkin’s solution.