Evaluation of pineapple waste crude extract in improving growth performance and resistance to Aeromonas hydrophila in Nile tilapia (Oreochromis niloticus)

The study on the antibacterial activity of fruit, peel, stem, and core of the pineapple, Ananas comosuscrude extract against Aeromonas hydrophilaand the effect of supplementing the diet with pineapple waste crude extract on growth performance and resistance against A. hydrophila in Nile tilapia, Oreochromis niloticuswere conducted. The best result of antibacterial screening was performed by disc method and the minimum inhibitory concentration was found in pineapple fruit extract with an inhibition zone of 7.96±0.20 mmand the minimum inhibitory concentration value was 125 mm per liter. The minimum bactericidal concentrationvalue was not observed in all parts of the pineapple extract at any concentration.Furthermore, Nile tilapia (2.34±0.82 g) werestocked in 12 aquariaand fed a diet supplementedwith 4different doses of pineapple peel extract(0, 1, 2and 3%)for 8weeks.The results revealed that thebest value of weight gain, average dailyweight gain, specific growth rate, feed conversion ratio, protein efficiency ratio and feed utilization efficiencywas observed in fish fed 1% pineapple peel extract andadditionally, the mortality of fish challenged by Aeromonashydrophilawas recorded for 10 days after the challenge. The result showed that fish-fed diet containing 1% pineapple peel extract had significantly the lowest mortality and the highest relative survival percentage.