Effect of Fibroblast Cells Injection on Healing of Diabetic Wounds and Improvement of Skin Elasticity and Thickness in Male Rats

Stem cells have a pivotal role in wounds healing; however, the mechanism of the healing effect of these cells is not still clear. The present study was carried out to investigate the effect of fibroblast cells on diabetic wound healing and skin thickness and elasticity in male rats. This research was done in Javid Biotechnology Laboratory in 2021.

In the present experimental laboratory study, fibroblast cells were isolated from the foreskin. Totally, 12 streptozotocin-induced diabetic male Wistar rats were divided into control (normal saline-treated) and fibroblast-treated rats. Wounds with a diameter of 0.8 cm were created via a biopsy puncture in the back of the mice. On days 7, 14, and 21 after treatment, the wound healing was evaluated via morphological observation, sonography, and elasticity assessment. Data were analyzed using one-way analysis of variance.

The healing rate and skin thickness and elasticity significantly increased in fibroblast cells treated group compared with control group. Compared with day 7, on days 14 and 21 after treatment, we observed significant increase in wound area skin gross elasticity (R2), recovery after deformation (R7) (p=0.008<0.01 and p=0.0007<0.001, respectively), and net elasticity (R5) (p=0.0008<0.001and p=0.009<0.01, respectively).

The results of the present study showed that foreskin derived fibroblast cells can accelerate diabetic wound healing by increasing the skin thickness and elasticity.