Efect of biosynthesized silver nanoparticles on Fusarium solani the cause of root rot bean's disease

The Safflower Carthamus with the scientific name tinctorius Carthamus L. belongs to the composite family and has antioxidant activity.Antifungal activity of silver nanoparticles synthesized by Safflower Carthamus extract was investigated against bean root rot disease caused by Fusarium solani in laboratory, greenhouse and field conditions.The synthesis of silver nanoparticles was evaluated by spectroscopic analysis (UV.Vis), Fourier transform infrared (FTIR), X-ray diffraction (XRD) and transmission electron microscopy (TEM).Factor disease severity were investigated in each pinto bean (Phaseolus vulgaris L.) plant.The results of the formation of silver nanoparticles were confirmed by changing the color of the silver nitrate solution to dark brown after adding the extract to the silver nitrate solution. The existence of the absorption maximum by UV-Vis analysis in the range of 415 nm is a proof of the synthesis of nanoparticles.Transmission electron microscopy showed the shape of nanoparticles to be spherical. XRD analysis showed the average size of nanoparticles to be 20 nanometers.In laboratory conditions, using the technique of mixing with the culture medium, the minimum growth inhibitory concentration (MIC) on the mycelial growth rate of the fungus was 200 ppm. PPM was evaluated by impregnating bean seeds with nanoparticles. The results of analysis of variance showed that the interaction effect between the concentration of different nanoparticles and the effect of the disease-causing fungus on the measured indicators is significant.