The effect of reducing physical activity after a period of endurance training on the expression of STIM1, Orai1 and MG29 genes in the plantaris muscle of male Wistar rats

Decreased physical activity in rodents has caused a decrease in muscle mass, cross-sectional area of myofibrils (CSA) and force production. The purpose of this study was to investigate the effect of reducing physical activity after a period of endurance training on the expression of STIM1, Orai1 and MG29 genes in the plantaris muscle of male Wistar rats.

The current research is experimental and basic. 16 male white desert rats, Wistar breed, weighing 250 ± 20 grams, were randomly divided into two groups. Control group - Spinal nerve ligation (SNL): This group included 8-week-old male rats whose three sciatic branches were tightly tied. The second group included 8 rats who received SNL after a period of endurance training. They did the endurance training protocol in the present study was for 4 days a week for 6 weeks. It increased from 10 meters per minute for 10 minutes in the first week to 17-18 meters per minute for 30 minutes in the fifth week. After the last training session, rats were anesthetized by intraperitoneal injection of ketamine and xylazine, and muscle tissue was extracted. To determine the significance of the difference between the variables, independent t-test was used, and SPSS-22 software was used to analyze the data with a significance level of P>0.05

The results of the present research showed that endurance training before SNL caused a significant change in the MG29 gene (P=0.07) and a significant increase in the Stim1 gene and Orai1 (P>0.05).Discussion and

Endurance training was able to modulate the expression disorder of these proteins in the state of neuropathy, which may be due to various mechanisms such as controlling oxidative stress factors and growth factors, in this way exerts its beneficial effects.