Effect of high intensity interval training on cardiomyocytes HSP70 and Caspase-3 gene expression levels in myocardial infarction male rat model.

The aim of this study was to investigate the effect of high intensity interval training on cardiomyocytes HSP70 and Caspase-3 gene expression levels in myocardial infarction (MI) male rat model. 

Thirty male Wistar rats (age: 16 wks, weight: 200 to 250 grams) were randomly divided into three groups of healthy control, MI control and MI training following induction of MI (verified by CK, LDH and CTnI as cardiac injury indices) via intraperitoneal injection of isoproterenol (100 mg/kg.day) within two consecutive days. HIIT was included on eight weeks (5 sessions per week), each session was consisted of 10 bouts of running occasions (each for 4 min) at 85 - 90% of vVo2max with 2 min rest intervals at 50 to 60% of vVO2max. The gene expression levels of HSP70 and Caspase-3 were evaluated using Real-Time PCR method and the data were analyzed using one-way analysis of variance and Tukey post hoc test at the significance level of p <0.05.

Cardiomyocytes HSP70 expression levels was lower in MI control than healthy control counterparts (p=0.001); However, in the HIIT group the expression levels of HSP70 and caspase-3 genes were respectively higher and lower compared to both of healthy control and MI control groups (p=0.001, p=0.001 respectively).

 It seems that HIIT could be efficient for inhibition of MI induced cardiac apoptosis. However further research is needed in this area because of study limitations and lack of similar evidence.