Alterations of miR-4772-3p and miR-3173-3p Expression in Tissue Compared to Normal Tissue by Real-time PCR

 In recent years, many studies have focused on the dysregulation of miRNA expression in cancer. These structures act as oncogenes and suppressors. In the present study, the expression levels of miR-3173-3p and miR-4772-3p were investigated in the tissue of glioblastoma multiforme (GBM) patients and healthy individuals.

 The present study was performed on 50 patients with GBM and 50 healthy individuals as the control group. Real-time PCR technique was used to determine the tissue levels of miR-3173-3p and miR-4772-3p. The biomarker evaluation of miR-3173-3p and miR-4772-3p was assessed using ROC curve analysis.

 The expression level of miR-4772-3p was higher in women with GBM (P-value = 0.03). Increased concentration of miR-3173-3p in tissue of GBM patients showed a significant correlation with a final grade of disease progression (grades III and IV) (P-value = 0.007). ROC curve analysis and cut-off of 0.6939 showed that miR-4772-3p could detect the presence of malignant GBM with 88.89 sensitivity and 100.0 specificity.

 The present study reveals the potential oncogenic role of miR-4772-3p. Moreover, the progression of glioblastoma cancer in the final stages can be assessed by measuring the level of miR-4772-3p in patients’ tissue.