Validation of the analysis method of 25 pesticide residues in rice using LC-MS/MS device (liquid chromatography-mass spectrometry)

In recent years, due to the increase in the use of pesticides in agricultural products and its entry into the human food chain, there has been an increase in concerns about the health of food among people. Therefore, the purpose of conducting this research is to validate the analysis method. 25 pesticide residues in rice using LC-MS/MS.

In this research, a simultaneous analysis method was set up to determine the amount of 25 commonly used pesticides in rice using a liquid chromatography device with a sequential mass spectrometer. Sample preparation steps were performed based on QuEChERS optimized method, using buffer extraction and purification based on d-SPE method. LC-MS/MS technique with electrospray ionization in positive mode was used for the simultaneous determination of 25 types of pesticides in a 20-minute run and in the MRM program.

The limit of quantification (LOQ) and limit of detection (LOD) for all pesticides were 25 and 8 ng/g, respectively. 30 samples of rice imported from Tehran were collected and analyzed. Out of the total number of 30 samples, 9 samples, i.e. 30% of the samples, were contaminated with Triazophos, Phoratesulfoxide, Fosthiazate, Formetanate, Fonofos, and Cyphenothrin toxins; Of these, 4 samples were higher than the MRL determined by the National Governor's Organization of Iran.