Numerical calculation of the lethality of becteria in bottled milk under cold plasma treatment

In recent years, cold plasma is one of the expected alternatives for post-harvest treatments and post-harvest management of products. A surface discharge plasma system was used for investigating the destruction time of Bacillus cereus, Bacillus coagulans, Bacillus stearothermophilus, and Clostridium botulinum in bottled milk. The simulation was performed by COMSOL a3.5 software for a two-dimensional geometry. The collected experimental data were simulated in COMSOL software. The k factor of microorganism deactivation data was used to validate the simulated data. Results showed that the production of reactive oxygen species during plasma treatment increases with time and extends to the entire container. The concentration of reactive oxygen species (at the output of the plasma probe) at the beginning of the production was high, and at the end when they leave the free surface of the milk, the concentration decreased. Increasing the initial temperature of milk sample, from 50 to 80℃, can cause significant changes in the amount of ozone from 125 mol/m3 to 266 mol/m3, respectively (p <0.05). However, voltage changes in these two temperatures did not show a significant effect on ozone concentration. Also, immediately upon the initiation of plasma treatment, plasma destruction begins where the concentration of active species is higher. It is shown that among the four studied bacteria, Bacillus stearothermophilus has the highest resistance against cold plasma, and after that other bacteria have shown similar resistance. Finally, it can be concluded that the deep plasma treatment in bottle can make it possible to overcome the surface limitation of cold plasma treatment.