Assessment of the expression of long noncoding RNA RMST following induction of neuronal differentiation of NT2 pluripotent embryonic carcinoma cells

The majority of neurogenesis is terminated soon after birth and in adults new neurons generated only in the sub ventricular zone and the hippocampus accordingly neurodegenerative disorders and nerve damage are vital problem. Although long non-coding RNAs (lncRNAs) are involved in several cellular process, their function in the regulation of neurogenesis should be further clarified. In this regards, this study aim to analyze expression levels of LncRNA RMST during neuronal differentiation of human pluripotent embryonal carcinoma NT2 cells. In this study, human pluripotent embryonal carcinoma NT2 cells were neurally differentiated though retinoic acids and a cocktail of neural. The expression levels of neural markers were analyzed by RT-PCR. During neural differentiation of NT2 cells, the expression levels of neural specific markers NSE and MAP2 were observed (***P