Chemical and antimicrobial properties of a flavonoid extracted from the Cleome turkmena Bobrov

In the process of extracting chemical compounds from the extract of   Cleome turkmena Bobrov, a flavonoid with a high weight percentage was extracted as pure green crystals.  The compound was purified using several chromatographic and recrystallization methods and identified by crystallography and 1H, 13C NMR and it was determined that this flavonoid is Salvigenin with the scientific name 5-hydroxy-4',6,7-trimethoxyflavone. Also, according to the lot of reports about the antibiotic properties of flavonoids, the effect of Salvigenin on inhibiting the growth of several bacteria and fungi was investigated by minimal inhibitory concentration (MIC) and disk diffusion (DD) methods.  The best inhibition against Bacillus pumilus with MIC value of 64 µg/ml. In disk diffusion method, best result was recorded against Salmonella typhi.