The effect of interval training and Nano selenium supplementation on the expression of testicular HSP70 and PGC-1α genes in dexamethasone induced rats

Exercise Training and selenium is an effective way to strengthen the immune system. The purpose of this research is the effect of interval training and nano selenium supplementation on the expression of testicular HSP70 and PGC-1α genes in dexamethasone induced rats. 40 Wistar rats aged 10 weeks were randomly divided into 5 groups of 8 (healthy, under dexamethasone induction (patient), patient + interval training, patient + Nano selenium supplement, patient + interval training + consumption Nano selenium supplement) were divided. The exercise program consisted of 5-12 repetitions of 1-minute running on the rodent treadmill with an intensity of 24-30 m/min, equivalent to 75-100% of maximal oxygen consumption, with active rest intervals of 75 seconds. These exercises were performed six days a week for four weeks. The prepared selenium nanoparticle solution, 100 mg in 250 nm size, was given to mice by gavage every other day. Three-way analysis of variance was used to determine the difference in variables between groups at a significant level (P<0.05). All statistical calculations were done using Excel and SPSS version 25 software. The results obtained from the research showed that interval training led to a significant increase in HSP70 (P<0.05). Also, the expression of PGC-1α gene in the group of Nano selenium supplementation and interval training led to a significant increase (P<0.05). According to the present study, it seems that interval training and Nano selenium supplementation leads to a significant increase in HSP70 and PGC-1α, which plays an important role in improving the immune function of patients with weak immune system.