biocompatibility investigation of hydroxyapatite/chitosan nanocomposites doped by metal oxides

Nano Hydroxyapatite (nHA) composites have been widely used in bone regeneration due to their unique properties. In this paper, doped nHA composites with doping of MgO, ZnO, and CeO2 metal oxides into nHA structure were successfully attained using precipitation method. Also, chitosan (CS) was added to the nHA composites to improve the biocompatibility. As the results showed, doping the metal oxides into the nHA structure led to the change of the lattice parameters in nHA structure. Finally, bioactivity of the composites was investigated by MTT assay by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide. MTT assay is shown that doped-nHA structure gives acceptable cytotoxicity against the MG-63 cell.