The Effect of Avicennia marina Flavonoids on Bax, Bcl-2 and Stress Oxidation Indicators of Epididymis Sperm in Type 1 Diabetic Rats

Diabetes affects the reproductive system and causes fertility disorders. Regarding the anti-oxidant and hypoglycemic properties of A. marina, the aim of this study was to determine the effect of total flavonoid contents on Bax, Bcl-2 level, and stress-oxidative stress indices of epididymis sperm in type 1 diabetic rats. In this experimental study, 32 male Wistar rats were divided into control, control diabetic, and two diabetic treated groups. The last two groups received 50 and 100 mg / kg Flavonoids in A. marina leaf for 30 days. At the end of the treatment period, sperms were extracted from the epididymis. Then Bax, Bcl-2, malondialdehyde, superoxide dismutase, glutathione peroxidase, catalase, and HOdG levels in sperm samples also FBS were measured by ELISA method. According to the results, the levels of Bcl-2, superoxide dismutase, glutathione peroxidase and catalase in sperm samples of diabetic rats treated with 50 and 100 mg / kg flavonoid concentrations of A. marina in Comparison with the control group, the diabetic was significantly increased, and the Bax, malondialdehyde and HOdG levels decreased significantly, Depending on the injectable dose (p <0.05). Flavonoid administration of A. marina leaves decreased apoptosis and stress-oxidative stress in spermatozoa of type I diabetic rats.