An overview of the importance and classifificatition of Real-Time PCR technique and its applicatition in food industry

The progress in the field of molecular technology and biology (caused to accelerate the correct identification of closely related species in a raw material and product) led to the frequency of molecular tests for wide applications such as genetic fingerprinting for identification, diagnosis of infectious diseases, food sensitivities and fraud detection in in the food industry it seems necessary. Certainly, using traditional methods (based on culture) to detect diseases, antibiotics, and harmful microorganisms in the food industry will be costly and take a lot of time. That's why modern molecular methods like RT-PCR were brought in. They can quickly and accurately determine the specific species that cause infections and assess the food's safety and quality. In this new method, fluorescent molecules are used to detect the gene amplification process. This allows for accurate measurement of gene expression levels with minimal risk of contamination. This technique is highly sensitive and specific, providing reliable data. So, it can be used instead of regular PCR. This article talks about the different methods of RT-PCR, why it's important, and how it can be used to detect allergens in food, like gluten. It also mentions how it can help uncover cases of food fraud.