Callus induction of different explants in Salsola tragus L.

Native plants to desert ecosystems, have unique features to ensure their survival in harsh conditions. Choghan(local name for Salsola tragus) is an ancient desert plant, have used for medicinal properties, and was the onlywashing plant in many parts of Iran. Urban culture and supply all kinds of chemical detergents have led to theforgetfulness of the capacities of national and patriotic detergent plant. Identify and preserve of such valuableplants that is resistant to harsh conditions and generally are halophyte, can be exploited in therapeuticapplications, detergent plant production programs, sustainable agriculture, environmental protection anddesertification. In order to study the response of callus initiation of this plant tissues, seeds were sterilized andplanted on MS medium. After two days, explants of root, stem and leaf were cultured on MS medium withNAA hormone with three levels: 0, 5 and 10 mg per liter. After three weeks, callus formation was evaluated.The results showed that the best explants for good callus induction, were the explants of leaf and then stem in16 hours of light with an intensity of 3000 lux at a concentration of 5 mg of the NAA.