Evaluation of Sucrose Benefits to Tuberization in ‘Sante’ Potato Cultivar in vitro

Sucrose plays a significant role in the process of microtuberzation in Potato. Sucrose acts as a crucial modifier, influencing the growth and development of microtuber in potato. Understanding the role of sucrose in microtuberzation is vital for improving potato cultivation practices and enhancing crop productivity. In this experiment, different concentrations of sucrose (3, 6, 9, 12, and 15%) were investigated to determine their effects on the microtuberization of the potato cultivar ‘Sante’. The findings revealed that higher concentrations of sucrose (12 and 15%) inhibited the microtuberization percentage, microtuber number, and shoot growth. The tuberization percentage reached its maximum value (100%) when exposed to a sucrose concentration of 9%. Additionally, the microtuber number per explant was observed to be 2, whereas it was 4.6 per vessel. However, the use of a 12% sucrose concentration resulted in the highest microtuber diameter (5mm), as well as the highest fresh weight (120 mg) and dry weight (26 mg) of microtubers and microtuber yield (FW: 752 mg; DW: 170 mg). On the other hand, the highest explant fresh weight (44 mg) and dry weight (9.5 mg) was observed in the 15% sucrose concentration. The allocation of biomass was influenced by the sucrose concentration, with higher concentrations leading to a greater allocation of biomass to the microtuber and explant rather than the shoot. Based on the outcomes obtained, it can be concluded that the optimal treatment for microtuber production of the potato Sante cultivar is a sucrose concentration of 12%.