Haematological changes and molecular detection of Anaplasma phagocytophilum in tick infested dogs in Isfahan Provice,Iran.

In this study, 100 blood samples of tick-infested dogs in Isfahan were collected and examined for molecular detection by Nested polymerase chain reaction (Nested PCR) method. Seven blood samples were found to be infected with Anaplasma phagocytophilum and their results were positive. Hematological studies of positive samples were showed that, in one of them, severe anemia, low hematocrit and neutrophillia without leukocytosis was seen.In four of them, anemia and neutrophilia (in 2 of them had leukocytosis also) was seen. and in two other positive samples, only neutrophilia was seen. In morbidity of the Anaplasma phagocytophilum infection in tick infested dogs, our examination and stastical analysis of data reveals that, age and contamination of the environement were affected but the genus were not affected the incidense of the disease.This study were conducted in four seasons of one year and it was done by the first time in Esfahan,middle province of Iran.