A study of antibiotic resistance of Escherichia coli isolated from urinary tract infection

Urinary tract infection (UTI) is one of the most common infectious diseases diagnosed in patients. Escherichia coli (E.coli) has been documented to be the most important pathogen associated with symptomatic tract infections. The aim of this study was to determine the local prevalence of bacterial and strains antimicrobial resistance of E.coli isolated from patients with UTI by reason increasing use of antibiotic and antibacterial resistance. In order to isolate and identify bacterial agents causing UTI and to indentify it’s sex and age distribiution, in a cross- sectional study, a total of 2101 midstream urine samples “through simple random sampling method” were analyzed for isolation and identification of bacterial isolates as per standard methods. Susceptibility tests were performed with disc diffusion tests using the Kirby-Bauer method on E.coli isolated from 233 cases with UTI aged from 3.5 month through 90 years with an average of 16.52±21.22 years. All data were analyzed with SPSS for windows. Out of which 353 samples were found to be significant bacteriuria (105 Cfu/mL) and remaining 1748 samples were either non-significant bacteriuria or very low bacterial count or sterile urine. In the present study, out of 353 isolated uropathogens the most common isolate was E.coli (66%) followed by staphylococcus sp (21.8%) klebsiella pneumoniae (7.93%), proteus vulgaris (3.4%), pseudomonas aeruginosa (0.84%). The UTIs were found to most frequently in female (78.5%) than male (21.5%) (P=0.000). The isolated E.coli showed resistant to amoxycilin (100%), ampicillin (99.1%) and sensitive to ciprofloxacin (85%) and ceftizoxime (60.1%). This study suggests that in emergency cases before the result of antibiogrom test is determined, the best choices to begin empiric treatment are cipro floxacin and ceftizoxime. amoxicillin and ampicillin are quite ineffective to treat these infections.