Effects of Melatonin on histopathological changes after experimental ovarian torsion -detorsion in cat

During the detorsion of a torsioned ovary, oxidant agents are released andmelatonin as an antioxidant can reduce ischemia. We studied the histopathologicalchanges after using melatonin on experimental torsioned ovary in cat.

The aim of this experimental study was to investigate the effects ofMelatonin on histopathological changes in torsion – detorsion injury in cat ovary.

An adnexal torsion – detorsion model was created by using 20adult cats randomly divided equally in to 2 groups of Saline and Melatonin. Ischemia wasinduced by iathrogenic 360° clockwise torsion of the cat adnex for 3 hr. Reperfusionwas achieved for 3 hr. Melatonin or saline were injected intra peritoneally (10mg/kg) 30min before ovarian detorsion in both groups. After 3 hr of ovarian detorsion, ovariantissue was removed and fixed in 10% formalin solution, embedded in paraffin andevaluated for ischemic indices.

Histological examination showed a significant improvement in ovarianmorphology in the melatonin treated cats. Edema and vasoconstriction in saline groupwere more severe than Melatonin group (p-value = 0.009). Hemorrhage and leukocyteinfiltration were also more obvious in saline group (p-value 0.0018)

Our results demonstrated that Melatonin administration reduced ovarianhistopathological damage due to oxidative injury associated with torsion.