The Study of Dose- Response Mitogenic Effect of L-dopa on the Human Periodontal Ligament Fibroblasts Cells

Statement of Problem: Avulsion is one of the most serious emergencies in dental office. Avulsed teeth should be stored in a medium that supports the periodontal ligament cells viability. In some clinical situations, preserving media, contained growth factors and mitogenic products may be used for repair of traumatized (Periodontal Ligament) tissues. It has been previously reported that levodopa (L-dopa) accelerates healing by increasing the growth hormone level. In this study, the local effect of L-dopa, on human periodontal ligament fibroblast (HPLF) cells was evaluated. Samples were taken from impacted or semi-impacted third molar teeth, which didn’t show inflammatory reaction. The cells obtained from periodontal ligament of these teeth, were cultured in appropriate medium. The passage number between 3-6 was taken for further experiments. The viability of HPLF cells, which treated, by L-dopa were evaluated by trypan blue dye exclusion and neutral red assay. Results indicate that low concentration of L-dopa produces significant increase in number of these cells in comparison with control group. These results confirmed previous study about direct action of L-dopa on the viability of HPLF cells. On the basis of this study and previous reports, presence of L-dopa in preserving media may be useful in increasing the self-life transferring HPLF cells.