Biocompatibility comparison of two different sealers AH26 and Apexit: In-vivo animal model

There are a few conditions that prove a sealer to be suitable for use in practice. One of the important factors in a good sealer is its biocompatibility. The purpose of this in-vivo study was to compare the biocompatibility of two types of endodontic sealers; AH26 and Apexit by using animal model. A total of eleven white rats were included in this interventional in-vivo study. After sedation of rats, two sealers (AH26 and Apexit) were implanted in the subcutaneous tissue of each rat. AH26 was implanted in two sites of subcutaneous tissue in the left side and Apexit was implanted in two sites in the right side and the control site was designated in between them. In control group, the incisions were made similar to AH26 and Apexit groups and sutured without insertion of any material. Biopsies were taken from five rats after three days and from the other six rats after thirty days. Histological specimens were studied by pathologist and findings were reported. Data was analyzed using appropriate statistical procedures as well as Mann–Whitney and Kruskal-Wallis tests in order to report the significance level Although Apexit caused more inflammation compared to AH26, after 3 and 30 days, but such difference was not statistically significant (P>0.05). However, both sealers initiated mild to moderate inflammation when judged against control group and the difference was statistically significant (P<0.05). Even though AH26 and Apexit cause inflammation in subcutaneous tissues of rats but they have moderate biocompatibility as the inflammation was graded two in this study according to the pathologic report.